Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation.
نویسندگان
چکیده
منابع مشابه
Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation.
The rate of appearance of duplex DNA renaturation, measured with single strand specific nuclease, deviates significantly from a second order reaction. Measurements reported in paper I of this series indicate an inhibition in the rate of reassociation of single strand tails on partially reassociated molecules by a factor of at least two. Equations are derived that describe the observed form of r...
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The reassociation kinetics of Escherichia coli DNA were measured by S1 nuclease resistance and hydroxyapatite binding. While the reaction assayed by hydroxyapatite displays second order kinetics, the S1 nuclease measurements follow a non-second order from, as previously reported by Morrow (Ph.D. Dissertation, Stanford University. 1974). Much of the reaction measured with S1 nuclease occurs betw...
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The rate of reaction of excess double-stranded bacteriophage phiX174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E. coli RNA labeled in vivo. RNA and DNA fragment lengths were held approximately equal. For each case it was shown that in DNA excess the rate constant for R...
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Conditions are described for effecting deoxyribonucleic acid renaturation and deoxyribonucleic acid-ribonucleic acid hybridization in formamide solutions at low temperatures. High specificity and rates of reaction can be achieved by appropriate choice of the formamide and salt conmalian nucleic acids. centrations and the temperature. Reaction conditions can be deduced from the relationship betw...
متن کاملReassociation kinetics of non-histone-bound DNA sites.
DNA-binding non-histone proteins (NHP) from rat liver have been prepared by utilizing their affinity toward phosphocellulose. The specifity of the DNA binding sites was investigated by the reassociation kinetics of the protein-bound DNA. Isolated protein-bound DNA (350 nucleotides) reassociated with a a-fold increase in the content of middle repetitive sequences as found in the whole rat genome...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1976
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.73.2.415